More Protein

Well I pressed ahead and cultured 7 liters of my protein. We use a arabanose induced chaperone before we induce with IPTG to help folding. After harvesting I placed in a Ni resin, because we have a His tag. In all I finished around 5:30 am. I was able to get home, get a hour cat nap, shower, and get back for a meeting. ... What an all nighter. Though all researchers know this is par for the course.